Koffas Research Group

Network and Metabolic Engineering

Rensselaer Polytechnic Institute

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Welcome to the Koffas Research Group home page!

News:

Dr. Koffas is now on the Editorial Board for the following journals: Biotechnology Journal, Biochemical Engineering Journal, Genes


Dr. Koffas is now Associate Editor for Scientific Reports.


Recent Publications, 2017

Group Picture


Koffas Group, July 2014.

From left: Dr. Mattheos Koffas, Yu Xiu, Shannon Collins, Wenqin He, Victoria Vernacchio, Emily Mazeau, Asher Williams, Dr. J. Andrew Jones, Karolina Kalbarczyk, Daniel Kim, Cliff Morrison, Kamil Gedeon, Daniel Lachance, Dr. Brady Cress, Jake Englaender, Reeve Aguayo, Louis-Xavier Barrette, Jordanne Gizzarelli, John Hordines, Krystyna Farrell, Quentin Leitz, Omar Khalidi.



Recent Publications

1. Wang, H., He, W., Jiang, P., Yu, Y., Lin, L., Sun, Z., Koffas, M., Zhang, F., & Linhardt, R.J. (2017). Construction and functional characterization of truncated versions of recombinant keratanase II from Bacillus circulans. Glycoconj J, 34, 643-649. doi: 10.1007/s10719-017-9786-3.
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2. He, W., Zhu, Y., Shirke, A., Sun, Z., Liu, J., Gross, R.A., Koffas, M.A.G., Linhardt, R.J., & Li, M. (2017). Expression of chondroitin-4-O-sulfotransferase in Escherichia coli and Pichia pastoris. Appl Microbiol Biotechnol, 101, 6919-6928. doi: 10.1007/s00253-017-8411-5.
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3. Williams, A., He, W., Cress, B.F., Liu, X., Alexandria, J., Yoshizawa, H., Nishimura, K., Toida, T., Koffas, M.A.G., & Linhardt, R.J. (2017). Cloning and Expression of Recombinant Chondroitinase ACII and Its Comparison to the Arthrobacter aurescens Enzyme. Biotechnology Journal. doi: 10.1002/biot.201700239.
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4. Jang, S., Jang, S., Xiu, Y., Kang, T.K., Lee, S.-H., Koffas, M.A.G., & Jung, G.Y. (2017). Development of Artificial Riboswitches for Monitoring of Naringenin In Vivo. ACS Synthetic Biology. doi: 10.1021/acssynbio.7b00128.
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5. Jones, J.A., Vernacchio, V.R., Collins, S.M., Shirke, A.N., Xiu, Y., Englaender, J.A., Cress, B.F., McCutcheon, C., Linhardt, R.J., Gross, R., & Koffas M.A.G. (2017) Complete biosynthesis of anthocyanins using E. coli polycultures. mBio, 8(3), e00621-17. doi: 10.1128/mBio.00621-17.
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6. Xiu, Y., Jang, S., Jones, J.A., Zill, N.A., Linhardt, R.J., Jung, G.Y., Yuan, Q., & Koffas, M.A.G. (2017) Naringenin-responsive riboswitch-based fluorescent biosensor module for Escherichia coli co-cultures. Biotechnology and Bioengineering, 114(4), 2235-2244. doi: 10.1002/bit.26340.
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7. Englaender, J.A., Zhu, Y.Y., Shirke, A.N., Lin, L., Liu, X., Zhang, F., Gross, R.A., Koffas, M.A.G., & Linhardt, R.J. (2017). Expression and secretion of glycosylated heparin biosynthetic enzymes using Komagataella pastoris. Appl Microbiol Biotechnol, 101, 2843-2851. doi: 10.1007/s00253-016-8047-x.
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8. Ito, T., Murai, M., Ninokura, S., Kitazumi, Y., Mezic, K.G., Cress, B.F., Koffas, M.A.G., Morgan, J.E., Barquera B., & Miyoshi, H. (2017). Identification of the binding sites for ubiquinone and inhibitors in the Na+-pumping NADH-ubiquinone oxidoreductase from Vibrio cholerae by photoaffinity labeling. J Biol Chem, 292(19), 7727-7742. doi: 10.1074/jbc.M117.781393.
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9. Cress, B.F., Leitz, Q.D., Kim, D.C., Amore, T.D., Suzuki, J.Y., Linhardt, R.J., & Koffas, M.A. (2017). CRISPRi-mediated metabolic engineering of E. coli for O-methylated anthocyanin production. Microbial Cell Factories.
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10. Englaender, J.A., Jones, J.A., Cress, B.F., Kuhlman, T.E., Linhardt, R.J., & Koffas, M.A. (2017). Effect of Genomic Integration Location on Heterologous Protein Expression and Metabolic Engineering in E. coli. ACS Synthetic Biology.
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11. He, L., Xiu, Y., Jones, J.A., Baidoo, E.E., Keasling, J.D., Tang, Y.J., & Koffas, M.A. (2017). Deciphering flux adjustments of engineered E. coli cells during fermentation with changing growth conditions. Metabolic Engineering.
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12. Whitaker, W.B., Jones, J.A., Bennett, K., Gonzalez, J., Vernacchio, V.R., Collins, S.M., Palmer, M.A., Schmidt, S., Antoniewicz, M.R., Koffas, M.A. & Papoutsakis, E.T. (2017). Engineering the Biological Conversion of Methanol to Specialty Chemicals in Escherichia coli. Metabolic Engineering. doi:10.1016/j.ymben.2016.10.015.
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13. Shirke, A.N., Su, A., Jones, J.A., Butterfoss, G.L., Koffas, M.A., Kim, J.R., & Gross, R.A. (2017). Comparative thermal inactivation analysis of Aspergillus oryzae and Thiellavia terrestris cutinase: Role of glycosylation. Biotechnology and Bioengineering. doi:10.1002/bit.26052.
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